Advanced organic chemistry of nucleic acids

Physical chemistry of nucleic acids.

The Watson-Crick double helix of DNA was first revealed in 1953. Since then a wide range of physical chemical methods have been applied to DNA and to its more versatile relative RNA to determine their structures and functions. My major goal is to predict the folded structure of any RNA from its sequence. We have used bulk and single-molecule measurements of thermodynamics and kinetics, plus var...

Understanding nucleic acids using synthetic chemistry.

This Account describes work done in these laboratories that has used synthetic, physical organic, and biological chemistry to understand the roles played by the nucleobases, sugars, and phosphates of DNA in the molecular recognition processes central to genetics. The number of nucleobases has been increased from 4 to 12, generating an artificially expanded genetic information system. This syste...

Twentyfourth Symposium on Nucleic Acids Chemistry Held

The Chemistry of the Nucleic Acids and Nucleoproteins

Isolation of nucleoproteins.-Many methods for isolating nucleoproteins involve a stage, either during the extraction or more generally in the precipitation process, which is relatively drastic and may produce an alteration in the chemical and physical properties of the nucleoprotein ; thus the majority of preparations involve precipitation of the extracted material with hydrochloric or acetic a...

Iodination of nucleic acids in organic solvents with iodine monochloride.

Iodine monochloride, labeled with 1z51, iodinated ribonucleic acid and deoxyribonucleic acid dissolved as hexadecyltrimethylammonium salts in anhydrous dimethylformamide or N-ethyl acetamide. In the case of RNA (polyuridylate, polycytidylate, and soluble RNA) 5-iodouridine S/-phosphate, 8-iodoguanosine 5’-phosphate, and 5-iodocytidine 5’-phosphate are found. After 24 hours of reaction, 32% of U...